gene transfer technique meaning in English
基因转移技术
Examples
- Establishment of conditionally immortalized rat cochlear greater epithelial ridge and lesser epithelial ridge cell lines using a retrovirus - mediated gene transfer technique
大鼠耳蜗大上皮嵴及小上皮嵴细胞永生细胞系的建立 - Now it has been one of the most important aquatic products in the freshwater cultivation . however , this prawn ca n ' t survive at a water temperature lower than 14c , which has seriously limited its cultivation expanding . in order to obta in a new breed of this prawn with increased cold - resistance , we investigated the cloning of a synthetic gene ( sbwafp ) based on the primary sequence of the mature spruce budworm ( choristoneura fumiferana ) antifreeze protein ( sbwafp ) and the integration of sbwafp into the embryo genomes of giant freshwater prawn by spermatophore - microinjection ( smi ) , a sperm - mediated gene transfer technique
本研究的特色和创新之处在于，针对罗氏沼虾不耐低温，但体型相对较大，精荚明显的特点，首次将目前已知具有最强抗冻活性的云杉卷叶蛾( sprucebudworm ， choristoneurafumiferana )抗冻蛋白( sbwafp )基因( sbwafp ) ，通过精子介导的转基因技术整合到罗氏沼虾的胚胎中，以期培育出耐低温的罗氏沼虾新品系。 - Using ex vivo gene transfer technique , exogenous gene was introduced to the liver graft during cold preservation and express locally in the graft . the effect of inhibition of rejection and inducing liver graft tolerance was observed . through this study , the possibility of achieving graft tolerance by gene transfer without routine immunosuppressive drugs was explored
我们采用adeasy腺病毒载体系统，自行构建携带huctla4 - ig的重组腺病毒，通过先体外后体内( exvivo )基因转移技术，于供肝冷保存时，将该治疗基因导入大鼠移植肝，使其于移植肝局部表达，观察其抑制排斥反应，诱导大鼠移植肝免疫耐受的作用。